SUMMARY: This article talked about using soil eutardigrades and bdelloid rotifer primers to quickly and easily identify these organisms directly. Due to the fact that there are so many types of microorganisms in soil, many of which are still unidentified and unable to be cultured in a lab, creating an efficient way to process large numbers of microorganisms is crucial to microbiology research. This new method of sequencing genomes is significant in that it will allow scientists to efficiently observe microorganism population and biodiversity. Microorganisms play an important role in ecosystems and biogeochemical cycling. It is in our interest to understand how changing global temperatures, pollution, and other anthropogenic effects influence their growth.
LESSON COMMENTS: This article can show students how researchers use products of transcription to identify DNA sequences. The concepts needed to understand how primers work are: complementary bases, transcription, differences in RNA structure and DNA. This article can also illustrate the importance of sequencing genomes and identifying organisms. Environmental science topics that can be covered include: global warming, anthropogenic effects on ecosystems, importance of biodiversity, and population changes.
Robeson, M. S., Costello, E. K., Freeman, K. R., Whiting, J., Adams, B., Martin, A. P., & Schmidt, S. K. (2009). Environmental DNA sequencing primers for eutardigrades and bdelloid rotifers. BMC ecology, 9, 25. doi:10.1186/1472-6785-9-25